Metabolic cage systems are routinely used in rodent metabolic studies to obtain behavioral characteristics and whole-body metabolic data such as energy expenditure, movement, feeding and drinking. Most metabolic cage systems require individual housing of mice for a period of time. Mice prefer social contact when given the choice as evidenced by the observation that single housed mice have dysregulated serotonergic signalling activity, a marker of psychological stress1. In this study, we examined whether social isolation affects behavioral and metabolic parameters, by performing baseline measures before placing either a single mouse (SH) or two mice (DH) into Promethion® metabolic cages for a period of 8 days. To calculate measures, where appropriate, the data were divided by two for DH. We observed that DH mice had a higher water intake compared with SH. We did not observe, however, any differences in food intake in both the light and dark cycle, respiratory exchange ratio, energy expenditure or movement (X, Y, Z beam breaks) when comparing SH with DH. In addition, the percentage of time mice spent walking, still, or asleep did not appear to differ when comparing SH with DH. While we acknowledge that dividing DH data by 2 where appropriate, is an assumption and somewhat of a crude measure, EchoMRI® data collected immediately following the intervention period demonstrated that total body, fat and lean mass were similar when comparing SH with DH. Although these data are preliminary, they suggest that social isolation of C57BL/6 mice for 8 days does not significantly affect body mass, food intake, energy expenditure or movement compared with dual housing mice.